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Item type: Item , Use of filter papers to determine seroprevalence of Toxoplasma gondii among hunted ungulates in remote Peruvian Amazon(Elsevier, 2014) Aston, E.J.; Mayor, P.; Bowman, D.D.; Mohammed, H.O.; Liotta, J.L.; Kwok, O.; Dubey, J.P.Toxoplasmosis is a zoonosis caused by the protozoan Toxoplasma gondii, and it is found worldwide. To determine whether ungulates are reservoirs of T. gondii in an isolated and remote region of the northeastern Peruvian Amazon, antibodies to T. gondii were determined in 5 species of ungulates by the modified agglutination test (MAT). These animals were hunted by subsistence hunters along the Yavarí-Mirín River, in the northeastern Peruvian Amazon. Blood samples were collected by hunters on filter papers. For determination of T. gondii antibodies, blood was eluted from filter papers, and a titer of 1:25 was considered indicative of exposure to T. gondii. Antibodies to T. gondii were found in 26 (31.0%) peccaries (Pecari tajacu, Tayassu pecari), six (17.1%) brocket deer (Mazama americana, Mazama gouazoubira), and four (40.0%) lowland tapir (Tapirus terrestris). We also introduced a modification to the MAT protocol that allows the extraction of fluid samples from several types of laboratory-grade filter paper, thus enabling researchers to easily adapt their approaches to the materials presented to them.Item type: Item , Spatial and temporal genetic diversity and population structure of Hemileia vastatrix from Peruvian coffee plantations(Korean Society of Plant Pathology, 2021) Quispe-Apaza, C.; Mansilla-Samaniego, R.; Espejo-Joya, R.; Bernacchia, G.; Yabar-Larios, M.; López-Bonilla, C.Population genetic studies of Hemileia vastatrix have been conducted in order to describe the evolutionary dynamics of the pathogen and the disease epidemiology as consequence of changes in disease management and host distribution occurred in Peru after the 2013 epidemic. These analyses were performed by sequencing the internal transcribed spacers of the nuclear ribosomal DNA (rDNA-ITS) of H. vastatrix collected from two coffee growing areas in 2014 and 2018. H. vastatrix population showed high haplotype diversity (Hd = 0.9373 ± 0.0115) with a low nucleotide diversity (π = 0.00322 ± 0.00018). Likewise, AMOVA indicated that fungus population has behaved as a large population without structuring by geographical origin and sampling years (FST = 0.00180, P = 0.20053 and FST = 0.00241, P = 0.19693, respectively). Additionally, the haplotype network based on intraspecific phylogenetic analysis of H. vastatrix using Peruvian and NCBI sequences revealed that Peruvian ancestral haplotypes, which were maintained in time and space, would correspond to the reported sequences of the races II and XXII. This re-sult suggests that no substantial changes have occurred through time in Peruvian Hemileia vastatrix population.Item type: Item , First record of Anastrepha pseudoparallela Loew (Diptera: Tephritidae) infesting passion flowers in Peru(Universidad Nacional Mayor de San Marcos, 2020) Salazar-Mendoza, P.; Ninahuamán-Calderón, A.; Fernández, C.G.; Strikis, P.C.Several species of the genus Anastrepha Schiner are associated to different hosts and have a great economic importance in fruit crops of the Neotropical region. Ripe and unripe fruits of six passion flowers species were sampled in Oxapampa during 2016 and 2018. Larvae of Anastrepha pseudoparallela Loew (Diptera: Tephritidae) were detected infesting fruits of Passiflora ambigua Hemsl and Passiflora quadrangularis L. For the first time, this fruit fly species is recorded attacking fruits of passion flowers in Peru.Item type: Item , New quarantine cold treatment for medfly Ceratitis capitata (Diptera: Tephritidae) in pomegranates(De Gruyter, 2025) Quenta, E.C.; Huaynate, C.C.; Ruidias, M.O.; Arambulo, T.T.; Febres, G.The medfly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is one of the most important agricultural pests worldwide due to its impact on harvest and market restrictions for host fruits in several countries. Pomegranates (Punica granatum [L.]; Lythraceae) are sensitive to chilling injuries caused by existing quarantine cold treatments. This study aimed to establish a new cold-Treatment schedule at 5°C for fresh pomegranates by determining the required exposure time to mitigate C. capitata in the pomegranate 'Wonderful' variety. The third-instar larva was deemed the most cold-Tolerant instar and was used for this study. The combination of 5.2°C for 36 days achieved 100% mortality.Item type: Item , Biological cycle and morphological characterization of the developmental states of Tuthillia cognata (Hemiptera: Psyllidae) in camu camu(Instituto de Investigaciones de la Amazonía Peruana, 2023) Campos-Celestino, V.E.; Velazco Castro, E.V.; Guerra-Ambrosio, B.; Sanchez-Choy, J.The jumping louse, Tuthillia cognata (Hemiptera: Psyllidae) is a pest that causes severe deformations in the leaf buds of Myrciaria dubia (camu camu) in the Peruvian Amazon, therefore, a study was carried out with the objective of determining the morphological characteristics of each stage of development, the duration and longevity of T. cognata adults in young shoots of camu camu. The research was carried out under laboratory conditions (temperature: 26,9 ± 2°C, relative humidity: 6,6 ± 5% and 12:12 h Light: Dark) in Pucallpa, Peru. Morphological characteristics of each stage of development, duration and longevity of T. cognata adults in young shoots of camu camu were determined. The duration from egg to adult was 17,8 ± 0,6 (12 to 23) days and longevity for females was 38,6 ± 1,3 (37 to 40) days and for males 35,6 ± 1,5 (34 to 38) days.
